Approaches to measuring the activities of protein arginine N-methyltransferases.

1 Abbreviations used: [methyl-C]AdoMet, S-adenosyl-L-[methyl-C]methionine; [methyl-H]AdoMet, S-adenosyl-L-[methyl-H]methionine; aDMA, asymmetric x-N,N-dimethylarginine; AdoMet, S-adenosyl-L-methionine; AdoHcy, S-adenosylL-homocysteine; CID, collision-induced dissociation; DMA, dimethylarginine; DTT, dithiothreitol; ESI, electrospray ionization; ETD, electron-transfer dissociation; FPLC, fast performance liquid chromatography; GAR, glycineand arginine-rich; GST, glutathione S-transferase; HEPES, 2-[4-(2-hydroxyethyl)piperazine-1-yl]ethanesulfonic acid; HPLC, high performance liquid chromatography; LOD, limit of detection; LOQ, limit of quantitation; MS, mass spectrometry; MS/MS, tandem mass spectrometry; MMA, x-N-monomethylarginine; OPA, o-phthalaldehyde; PRMT, protein G 0G Ted M. Lakowski